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Double asterisks indicate the position for the second z-axis reconstruction directly below the first z-axis reconstruction. Single asterisks indicate the position for the first z-axis reconstruction directly below the x-y confocal image. These cells were then stained with phalloidin green and fluorescent streptavidin red. The macrophage contents evaluated by Mac-1 staining of the aorta root sections and F4/80 and CD80 double staining of aorta sections was reduced by Cd11b-cre Trpm2 deletion Fig. Therefore, you are first initializing an array of axes objects defaulting to. ax axes.Axes object or array of Axes objects. Help Online - Origin Help - Multiple Y Axes Graph.įirst, assigning y1 and y2 objects is unnecessary as you will never use them later on.

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Graphing data expressed as fold changes, or ratios.Help Online - Origin Help - Double-Y Column Graph.Help Online - Origin Help - Multiple Y Axes Graph.